3种银杏黄酮苷类化合物定量分析方法比较研究

钟媛媛, 王京辉, 陈晶, 王萌萌, 郭洪祝, 傅欣彤, 陈有根

中国药学杂志 ›› 2019, Vol. 54 ›› Issue (11) : 916-921.

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中国药学杂志 ›› 2019, Vol. 54 ›› Issue (11) : 916-921. DOI: 10.11669/cpj.2019.11.011
论著

3种银杏黄酮苷类化合物定量分析方法比较研究

  • 钟媛媛1,2, 王京辉1*, 陈晶1, 王萌萌1, 郭洪祝1*, 傅欣彤1, 陈有根1
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Comparative Study on Three Quantitative Methods for Determination of Flavonoid Glycosides in Ginkgo biloba Oral Preparations

  • ZHONG Yuan-yuan1,2, WANG Jing-hui1*, CHEN Jing1, WANG Meng-meng1, GUO Hong-zhu1*, FU Xin-tong1, CHEN You-gen1
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摘要

目的 为改进目前银杏叶类制剂黄酮醇苷成分的含量控制采用间接测定方式的现状,建立一测多评测定法以及标化对照提取物实现量值转移的测定方法,使得银杏黄酮苷类成分的控制更加准确可靠。方法 采用YMC-Pack ODS-AQ (4.6 mm×250 mm,5 μm)色谱柱;乙腈(A)-0.4%磷酸溶液(B),梯度洗脱,流速1.0 mL·min-1,检测波长360 nm;以芦丁为内参物,建立银杏叶口服制剂中5种黄酮醇苷[山柰酚3-O-芸香糖苷(KGR)、异鼠李素3-O-芸香糖苷(IGR)、山柰酚3-O-鼠李糖-2-葡萄糖苷(KRG)、槲皮素3-O-鼠李糖-2-O-(6-O-对羟基反式桂皮酰)-葡萄糖苷(QRcG)及山柰酚3-O-鼠李糖-2-O-(6-O-对羟基反式桂皮酰)-葡萄糖苷(KRcG)]“一测多评”含量测定方法,确定了5种黄酮醇苷对于芦丁的相对校正因子;对中国食品药品检定研究院发放的对照提取物以及自制银杏黄酮苷对照提取物中5种黄酮醇苷的含量进行了标定。结果 所建立的“一测多评”方法各检测成分的相对校正因子,在不同仪器、色谱柱、不同浓度及进样体积条件下重复性好,方法可行。标定了银杏叶对照提取物和制备的银杏黄酮苷对照提取物中黄酮苷的含量。3 种方法含量测定结果与外标法测定结果进行统计分析,结果显示,4种测定方法间无明显差异(P>0.05)。结论 4种检测方法均可有效、准确地对银杏叶多剂型口服制剂进行含量测定,一测多评法和对照提取物法实现了量值转移,解决无对照物质问题,为评价银杏叶口服制剂的质量提供了可行的方法。

Abstract

OBJECTIVE To establish a QAMS and a method for quantitative transfer of standardized control extracts to change the current status of controlling flavonoid glycosides in Ginkgo biloba preparations by means of indirect determination, thus making the control of flavonoid glycosides of Ginkgo biloba more accurate and reliable. METHODS The YMC-Pack ODS-AQ column (4.6 mm×250 mm, 5 μm) was adopted. The mobile phase consisted of acetonitrile-0.4% phosphoric acid and gradient elution was conducted at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 360 nm. Five kinds of flavonoid glycosides in various oral preparations of Ginkgo biloba were determined by single marker(QAMS)method. Rutin was used as internal reference standard and the relative correction factors(RCFs)of KGR, IGR, KRG, QRcG and KRcG to rutin were calculated. The ginkgo flavonoid glycosides extract control was then prepared. Two batches of Ginkgo biloba extract control were demarcated,one was laboratory homemade, and the other was issued by the National Institutes for Food and Drug Control (NIFDC). RESULTS The RCFs determined by different instruments and columns at different concentrations and injection volumes had good reproducibility. The contents of flavonoid glycosides in the prepared ginkgo flavonoid glycosides extract control and Ginkgo biloba extract control were calibrated. No significant difference was found among the results of the above-mentioned three methods and the external standard method (P>0.05). CONCLUSION The four methods can all effectively and accurately determine the contents of flavonoid glycosides in various oral preparations of Ginkgo biloba. QAMS and standardized extract control method may achieve quantitative transfer. They can solve the problem of lack of reference substances and provide a feasible method for evaluating the quality of Ginkgo biloba oral preparations.

关键词

银杏叶口服制剂 / 多剂型 / 黄酮苷类 / 定量研究 / 一测多评 / 银杏叶对照提取物 / 银杏黄酮苷对照提取物

Key words

oral preparations of Ginkgo biloba / multiple dosage form / flavonoid glycoside / quantitative research / QAMS / Ginkgo biloba flavonoid glycoside extract control / Ginkgo flavonoid glycoside extract

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钟媛媛, 王京辉, 陈晶, 王萌萌, 郭洪祝, 傅欣彤, 陈有根. 3种银杏黄酮苷类化合物定量分析方法比较研究[J]. 中国药学杂志, 2019, 54(11): 916-921 https://doi.org/10.11669/cpj.2019.11.011
ZHONG Yuan-yuan, WANG Jing-hui, CHEN Jing, WANG Meng-meng, GUO Hong-zhu, FU Xin-tong, CHEN You-gen. Comparative Study on Three Quantitative Methods for Determination of Flavonoid Glycosides in Ginkgo biloba Oral Preparations[J]. Chinese Pharmaceutical Journal, 2019, 54(11): 916-921 https://doi.org/10.11669/cpj.2019.11.011
中图分类号: R284   

参考文献

[1] Ch.P 2015. Vol Ⅰ(中国药典2015年版.一部)[S]. 2015:316.
[2] HE H H, DU X, JIN H H, et al. HPLC Fingerprint of Ginkgo biloba extracts and the structural identification of common peaks by LC-MS [J]. Chin J Pharm Anal(药物分析杂志),2017,37(6):1107-1112.
[3] LIU G X, LI H, ZHANG J. Rapid evaluation on quality control of ginkgo leaves extract and preparations by UPLC fingerprint [J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志),2017,23(1):64-68.
[4] ZHU S, XING B Q, MEI D, et al. Analysis on adverse drug reaction reports induced by Ginkgo biloba leaves extract injection from 200[J]. Chin J Pharmacoepidemiol(药物流行病学杂志),2017,26(1):50-53.
[5] LI S Q, ZHU J B, WU Y Z. Research progress of Ginkgo biloba extract for cardiovascular and cerebrovascular diseases[J]. Chin New Drugs J(中国新药杂志),2016,25(1):76-81.
[6] LIU G L.The pharmacological effect and clinical application of the Ginkgo biloba extract[J]. Shanghai Med Pharm J(上海医药),2016,37(3):8-22.
[7] WANG Y N, WANG J H, CHEN J, et al. Establishment of the UPLC fingerprints of Yinxingye tablets and the application in quality control[J]. Chin J Pharm Anal(药物分析杂志),2016,36(5):847-855.
[8] GE X H, LIU S X, REN T, et al. Advances research on chemical constituents, preparations and adverse reactions of Ginkgo biloba[J]. Drug Eval Res(药物评价研究),2017,40(6):742-751.
[9] LIU H, GAO H. Advances in research on pharmacological effects of Ginkgo biloba extract[J]. J Hubei Univ Sci Technol (Med Sci)(湖北科技学院学报:医学版),2015,29(3):259-262.
[10] KANG W. Study on chemical composition and medicinal use of Ginkgo biloba leaves [J]. China Health Ind(中国卫生产业),2014,11(2):194-195.
[11] YANG Y, ZHOU B, ZHAO W J. Analysis and consideration on Ginkgo folium event[J]. Chin Tradit Herb Drugs(中草药),2016,47(14):2397-2407.
[12] ZHAO Y Y,GUO H Z,CHEN Y G, et al. A comparative study and suggestion on relative correction factor produced by different methods in quantitative analysis of multi-components[J]. Drug Stand China(中国药品标准),2014,15(4):245-251.
[13] WANG J H, CHEN Y G, ZHAO Y Y, et al. UPLC Determination of 6 flavonol glycosides in Shuxuening injection[J]. Chin J Pharm Anal(药物分析杂志),2010,30(8):1574-1577.
[14] ZHAO Y Y, WANG J H, FU X Y, et al. Simultaneous determination of eleven flavonoid glycosides in Ginkgo biloba leaves and study on its fingerprint by ultra performance liquid chromatography method[J]. Chin Pharm J(中国药学杂志),2013,48(18):1583-1588.
[15] ZHAO Y Y, CHEN Y G, GUO H Z, et al. Chemical constituents of flavonoid glycosides in extract from Ginkgo biloba leaves used for injection [J]. Chin Tradit Herb Drugs(中草药),2013,44(15):2027-2034.

基金

国家科技重大专项课题-中药质量标准研究和信息化体系建设平台资助项目(2012ZX09304005)

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